publications
Publications from the lab
2022
Narayanan V, Bobbili KB, Sivaji N, Jayaprakash NG, Suguna K, Surolia A, Sekhar A. Structure and Carbohydrate Recognition by the Nonmitogenic Lectin Horcolin. Biochemistry. 2022 Feb 28. Access Article
Jain S, Sekhar A. Elucidating the mechanisms underlying protein conformational switching using NMR spectroscopy. Journal of Magnetic Resonance Open. 2022 Jan 10:100034. Access Article
2021
Jaladeep A, Varghese CN, Sekhar A. Measuring radiofrequency fields in NMR spectroscopy using offset-dependent nutation profiles. Journal of Magnetic Resonance. 2021 Sep 1;330:107032. Access Article
Madhurima K, Nandi B, Sekhar A. Metamorphic proteins: the Janus proteins of structural biology. Open biology. 2021 Apr 21;11(4):210012. Access Article
2019
Kumar A, Narayanan V, Sekhar A. Characterizing post-translational modifications and their effects on protein conformation using NMR spectroscopy. Biochemistry. 2019 Nov 4;59(1):57-73. Access Article
Munshi S, Subramanian S, Ramesh S, Golla H, Kalivarathan D, Kulkarni M, Campos LA, Sekhar A, Naganathan AN. Engineering Order and Cooperativity in a Disordered Protein. Biochemistry. 2019 Apr 19;58(19):2389-97. Access Article
Representative publications (before IISc)
(these should give you an idea of the NMR methods we routinely employ in the lab)
1) Rosenzweig R*, Sekhar A*, Nagesh J and Kay L E "Promiscuous binding by Hsp70 results in conformational heterogeneity and fuzzy chaperone-substrate ensembles" (2017) eLife, 6, e28030. (* equal contributors and joint corresponding authors) Link
2) Sekhar A, Rumfeldt J A O, Broom H R, Doyle C M, Sobering R E, Meiering E M and Kay L E "Probing the free energy landscapes of ALS disease mutants of SOD1 by NMR Spectroscopy" (2016) Proc. Natl. Acad. Sci. U.S.A., 113, E6939-E6945. Link
3) Sekhar A*, Rosenzweig R*, Bouvignies G and Kay L E "Hsp70 biases the folding pathways of client proteins" (2016) Proc. Natl. Acad. Sci. U.S.A., 113, E2794-E2801. (* equal contributors) Link
4) Sekhar A*, Rosenzweig R*, Bouvignies G and Kay L E "Mapping the conformation of a client protein through the Hsp70 functional cycle" (2015) Proc. Natl. Acad. Sci. U.S.A., 112, 10395-10400. (* equal contributors and joint corresponding authors) Link
5) Sekhar A, Rumfeldt J A O, Broom H R, Doyle C M, Bouvignies G, Meiering E M and Kay L E "Thermal fluctuations of immature SOD1 lead to separate folding and misfolding pathways" (2015) eLife, 4, e07296. (joint corresponding author) Link
6) Sekhar A and Kay L E "NMR paves the way for atomic level descriptions of sparsely populated, transiently formed biomolecular conformations" (2013) Proc. Natl. Acad. Sci. U.S.A., 110, 12867-12874. (Review) Link
7) Sekhar A*, Vallurupalli P* and Kay L E "Defining a length scale for millisecond-timescale protein conformational exchange" (2013) Proc. Natl. Acad. Sci. U.S.A., 110, 11391-11396. (* joint corresponding authors) Link
For Ashok's full list of publications, please click here.
Link to Ashok's google scholar profile.
