courses
Biomolecular NMR Spectroscopy
MB 305 (AUG) 3:0
Instructors: Siddhartha P Sarma and Ashok Sekhar
Basic theory of NMR spectroscopy. Classical and theoretical descriptions of NMR spectroscopy. Product operator formalism for description of multipulse homonuclear and heteronuclear NMR experiments. Multidimensional NMR spectroscopy. Description of basic homonuclear two-dimensional NMR experiments useful for structure determination of biological macromolecules. Experimental aspects of homonuclear NMR spectroscopy: data acquisition, processing and interpretation of 2D homonuclear spectra. Principles of heteronuclear NMR spectroscopy. Analysis of 3D and 4D heteronuclear isotope edited NMR pulse sequences. Introduction to relaxation and dynamic processes (chemical and conformational processes) that affect NMR experiments.
Reference books:
1) Protein NMR Spectroscopy Principles and Practice
2) J. Cavanaugh, Fair Brother, A.G. Palmer III & N. Skelton (1995), Academic Press.
3) Spin Dynamics - .M. Levitt (2000) John Wiley.
4) NMR of Proteins & Nucleic Acids Kurt Wuthrich, John Wiley (1986).
MB 305 (AUG) 3:0
Instructors: Siddhartha P Sarma and Ashok Sekhar
Basic theory of NMR spectroscopy. Classical and theoretical descriptions of NMR spectroscopy. Product operator formalism for description of multipulse homonuclear and heteronuclear NMR experiments. Multidimensional NMR spectroscopy. Description of basic homonuclear two-dimensional NMR experiments useful for structure determination of biological macromolecules. Experimental aspects of homonuclear NMR spectroscopy: data acquisition, processing and interpretation of 2D homonuclear spectra. Principles of heteronuclear NMR spectroscopy. Analysis of 3D and 4D heteronuclear isotope edited NMR pulse sequences. Introduction to relaxation and dynamic processes (chemical and conformational processes) that affect NMR experiments.
Reference books:
1) Protein NMR Spectroscopy Principles and Practice
2) J. Cavanaugh, Fair Brother, A.G. Palmer III & N. Skelton (1995), Academic Press.
3) Spin Dynamics - .M. Levitt (2000) John Wiley.
4) NMR of Proteins & Nucleic Acids Kurt Wuthrich, John Wiley (1986).
Relaxation Theory and Applications to Solution State Biomolecular NMR Spectroscopy
MB 316 (JAN) 3:1
Instructors: Ashok Sekhar
Chemical shift and dipolar coupling Hamiltonians, Redfield theory - derivation of the Redfield master equations for predicting relaxation rates and key assumptions, relaxation in homonuclear and heteronuclear two-spin IS systems, T1 relaxation, T2 relaxation, cross-relaxation, relaxation interference, a brief introduction to the origins of biomolecular dynamics and the concept of the conformational free energy landscape, utilizing relaxation rates for understanding biomolecular dynamics: the model-free approach, exchange-mediated relaxation: the chemical shift timescale and fast, slow and intermediate timescale exchange processes, experiments for estimating exchange parameters
Prerequisities: MB305 or knowledge of basic NMR theory, product operator formalism and its use in analysis of 2D and 3D NMR pulse sequences
Reference Books:
1) Arthur G. Palmer III, Wayne J. Fairbrother, John Cavanagh, Nicholas J. Skelton and Mark Rance, Protein NMR Spectroscopy,: Principles and Practice (Second Edition)
2) Josef Kowalewski and Lena Maler, 'Nuclear Spin Relaxation in Liquids: Theory, Experiments, and Applications' (Second Edition)
MB 316 (JAN) 3:1
Instructors: Ashok Sekhar
Chemical shift and dipolar coupling Hamiltonians, Redfield theory - derivation of the Redfield master equations for predicting relaxation rates and key assumptions, relaxation in homonuclear and heteronuclear two-spin IS systems, T1 relaxation, T2 relaxation, cross-relaxation, relaxation interference, a brief introduction to the origins of biomolecular dynamics and the concept of the conformational free energy landscape, utilizing relaxation rates for understanding biomolecular dynamics: the model-free approach, exchange-mediated relaxation: the chemical shift timescale and fast, slow and intermediate timescale exchange processes, experiments for estimating exchange parameters
Prerequisities: MB305 or knowledge of basic NMR theory, product operator formalism and its use in analysis of 2D and 3D NMR pulse sequences
Reference Books:
1) Arthur G. Palmer III, Wayne J. Fairbrother, John Cavanagh, Nicholas J. Skelton and Mark Rance, Protein NMR Spectroscopy,: Principles and Practice (Second Edition)
2) Josef Kowalewski and Lena Maler, 'Nuclear Spin Relaxation in Liquids: Theory, Experiments, and Applications' (Second Edition)